2) The book: It was a ritual in a painting or per usual to see Mary reading a book, after many controversy it was best said that the book was the old testament. It was more specifically known as the the book of Isaiah  where Virgin Mary was able to see the foretelling of her child. 

3) The lily: Is a representation of purity, its is known to be the centre object Angel Gabriel is holding. The lily was more common in paintings in Florence, Italy, due to their coat of arms corresponding with the red lily. On the other hand, places like Siena ( small part of Florence) included Angel Gabriel holding an olive branch to show purity. There was a huge range of what Angel Gabriel could be holding but they all signified the same aspect. 

4) The candle: There are two types of candles within the Annunciation, lit candles and unlit candles each signifying a new message. The unlit candles symbolised a life which has not yet be born, for example Christ. You are able to find the unlit candles on the Chandelier. On the other hand, the lit candles represented a specific journey in this Painting which was from the heavenly word to the world the painting is set in.  

5) The curtain: It was located in the same room as the candle and represents "revelation" meaning when pulled it will reveal an important or sacred image to the people. An example of this is, when Mary built a temple it was known to have a veil on it, this signifies that it is "holy of holies".

6) The angels: The angels played a huge role in Christian symboilsim, they adverted the way the Christians thought about Mary which was placed by Giotto. They enabled her to be "perceived" as a queen whom was close to mankind rather than a Theotokos who was known to be distant and far way from the human class. Angels had a new look in this painting along with new Renaissance art due to the development of the christian outlook which gave the angels a feminine feel which was light, heavenly and full of happiness.

7) Mary's throne:  Her throne is shown as if it was a "monument" and not part of the reality we are surrounded with. It symbolises her to be put in a more "divine dimension". This also contradicts with the "drape of honour" which is always behind Mary as per seen at the back of Emperor's in Imperial Rome.​

8) Saint Edimus: Was known as the great patron saint of Ascoli, within the painting he is shown holding a model of Ascoli ("the newly freed Ascoli") which has no major changes from what modern Ascoli looked like. He is also put in paintings because he is the saint of earthquakes, so he may be able to "protect" the city.

9) The Cucumber: Also known as the Gherkin was a great example of Christian symbolism because it symbolised Mary due to do the contradictions; it was mentioned in a quote from Isaiah. "And the daughter of Zion is left as a cottage in a vineyard, as a lodge in a garden of cucumbers, as a besieged city." The daughter of Zion meaning Mary was in relation with the garden of cucumbers.​